The use of mixed cultures has been criticized be cause it is difficult to demonstrate conclusively which microorganisms are responsible for the observed effects, how the introduced microorganisms interact with the indigenous species, and how these new associations affect the soil plant environment. Thus, the use of mixed cultures of beneficial micro-organ isms as soil inoculants to enhance the growth, health, yield, and quality of crops has not gained widespread acceptance by the agricultural research establishment because conclusive scientific proof is often lacking.
The use of mixed cultures of beneficial microorganisms as soil inoculants is based on the principles of natural ecosystems which are sustained by their constituents; that is, by the quality and quantity of their inhabitants and specific ecological parameters, i.e., the greater the diversity and number of the inhabitants, the higher the order of their interaction and the more stable the ecosystem. The mixed culture approach is simply an ef fort to apply these principles to natural systems such as agricultural soils, and to shift the microbiological equilibrium in favor of increased plant growth, production and protection.
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